PURE1A

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Application	PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
	Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
Features and Benefits	• High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run • Specific - will only bind IgG • Easy to use - antibody is eluted and desalted in a single step, ready to use • Gentle - avoids prolonged exposure of the antibody to low pH
Principle	Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.
Specificity	Capacity/Run (by Species) Human IgG 20-25 mg Mouse IgG1 8-12 mg Mouse IgG2a 10-15 mg Mouse IgG2b 6-10 mg Mouse IgG3 15-20 mg Rabbit IgG 8-12 mg Goat IgG 2-4 mg Bovine IgG 8-12 mg

usage	sufficient for 10 purifications
shipped in	wet ice
storage temp.	2-8°C